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Protein Chromatography
- Course Program |
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Sunday, May 13
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17:00 - 17:30 |
Registration |
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17:30 - 18:00 |
Introduction to the course |
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18:00 - 19:00 |
Lecture:
Downstream processing of biotechnological products |
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19:00 - |
Reception |
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Monday, May 14
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9:00 - 11:00 |
Lecture:
Introduction to chromatography, basic principles, modes of operation |
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11:00 - 12:30 |
Lecture:
The chromatographic workstation |
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12:30 - 13:30 |
Lunch break |
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13:30 - 16:00 |
Laboratory I:
Pulse response, determination of retention factor and HETP |
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16:00 - 17:30 |
Lecture:
Categories and properties of chromatographic media |
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Tuesday,
May 15
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8:30 - 11:00 |
Evaluation of Laboratory I data |
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11:00 - 12:30 |
Lecture:
Mass transfer effects - Fundamentals |
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12:30 - 13:30 |
Lunch break |
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13:30 - 15:30 |
Lecture:
Mass transfer effects - Applications |
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15:30 - 17:30 |
Laboratory II
Frontal analysis, DBC and scale-up parameters |
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Wednesday,
May 16
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8:30 - 11:00 |
Evaluation of Laboratory II data |
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11:00 - 12:30 |
Lecture:
Monoclonal antibody Protein A capture |
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12:30 - 13:30 |
Lunch break |
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13:30 - 14:30 |
Lecture:
Linear gradient elution theory |
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14:30 - 17:30 |
Laboratory III
Gradient elution, determination of retention factor vs. salt |
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Thursday,
May 17
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8:30 - 11:00 |
Evaluation of Laboratory III data |
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11:00 - 12:30 |
Lecture:
HIC and Protein/Surface Interactions |
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12:30 - 13:30 |
Lunch break |
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13:30 - 15:30 |
Lecture:
Determination and use of scale-up parameters |
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15:30 - 17:30 |
Laboratory IV
Gradient elution, HETP from gradient runs and optimization |
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19:00 - |
Course dinner |
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Friday,
May 18
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9:00 - 11:00 |
Evaluation of Laboratory IV data |
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11:00 - 12:30 |
Chromatographic Process Design Exercises |
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12:30 - 13:30 |
Lunch break |
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