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Protein Chromatography
- Course Description |
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The scope of the course is to provide insight in the application of
chromatographic theory with special
emphasis on the determination and use of key scale-up parameters.
The course will include both lectures and hands-on laboratories. The
lectures will cover an overview of downstream processing of
biotechnological products, the different modes of operation of
chromatography, the characteristics of chromatographic media, the
description of adsorption equilibria and mass transfer, the effects
of dispersion on chromatographic performance, frontal analysis and
linear gradient elution theories, and protein-protein and
protein-surface interactions. The laboratory sessions will comprise
pulse response experiments, the determination of retention factor
and HETP, frontal analysis and dynamic binding capacity experiments,
and linear gradient elution experiments. The experiments will be
carried out with typical chromatography media using AKTA Explorer
chromatographic workstations from GE Healthcare and will explore the
effects of particle size and chemistry, the size of the protein, and
the choice of operating conditions. The participants divided in
teams will set up the experimental runs, analyze the experimental
results, calculate performance metrics, determine scale-up
parameters, and present the results for discussion in a group
setting. Detailed course notes and spreadsheet-based tools for data
analysis will be provided. |
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Who Should Attend
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The course is aimed at bioprocess development engineers, separation
scientists, biologists, biochemists and technical managers having
some familiarity with downstream process development who want to
develop a deeper understanding of chromatographic processes and
their scale-up. Graduate students and separation scientists in
academia will also benefit from this course. |
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Lecturers
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Professor Giorgio Carta
Department of Chemical Engineering, University of Virginia,
Charlottesville, Virginia
Professor Alois Jungbauer
Department of Biotechnology, University of Natural Resources and
Applied Life Sciences
Vienna, Austria
Professor Erik Fernandez
Deptartment of Chemical Engineering, University of Virginia,
Charlottesville, Virginia
Graduate assistants will support the participants during the
laboratory and data analysis sessions |
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